山东净之源净化工程有限公司

  • 地址:济南市槐荫区鑫苑世家公馆2号楼2603室
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  • 无菌室的工作规程包括?
    来源:http://www.hnsfk.com 浏览: 发布日期:2018-05-14
    (1)无菌室灭菌。每次使用前开启紫外线灯照射30min以上,或在使用前30min,对内外室用5%石炭酸喷雾。
    (1) sterilization in the asepsis room. Before each use, turn on the ultraviolet lamp for 30min or 30min before use, and use 5% carbolic acid spray in the interior and exterior chambers.
    (2)用肥皂洗手后,把所需器材搬入外室;在外室换上已灭菌的工作服、工作帽和工作鞋,戴好口罩,然后用2%煤酚皂液将手浸洗2分钟。
    (2) after washing hands with soap, carry the required equipment into the outer room; replace the sterilized work clothes, work hats and work shoes in the outer room, wear a mask, and then wash the hand with 2% phenol soap for 2 minutes.
    (3)无菌室装修将各种需用物品搬进内室清点、就位,用5%石炭酸在工作台面上方和操作员站位空间喷雾,返回外室,5~10min后再进内室工作。
    (3) the decoration of the asepsis room will move all kinds of items into the inner room and place, with 5% carbonic acid on the top of the work table and the operator station space spray, return to the outer room, and then enter the inner room after 5 ~ 10min.
    (4)接种操作前,用70%酒精棉球擦手;进行无菌操作时,动作要轻缓,尽量减少空气波动和地面扬尘。
    (4) wipe the hands with 70% alcohol cotton balls before inoculation. When performing aseptic operations, the movements should be gentle, minimizing air fluctuations and surface dust.
    (5)工作中应注意安全。如遇棉塞着火,用手紧握或用湿布包裹熄灭,切勿用嘴吹,以免扩大燃烧;如遇有菌培养物洒落或打碎有菌容器时,应用浸润5%石炭酸的抹布包裹后,并用浸润5%石炭酸的抹布擦拭台面或地面,用酒精棉球擦手后再继续操作。
    (5) safety should be paid attention to in the work. If the cotton is caught in a fire, hold the hand tightly or put out with a wet cloth, do not blow it with your mouth so as not to expand the combustion. If there is a fungus culture, if it is sprinkled or shattered with a fungus container, the 5% carbonic acid soaked cloth is used to wipe the surface or ground with a 5% carbonic acid.
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