山东净之源净化工程有限公司山东净之源净化工程有限公司

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  • 生物安全柜标准操作规程
    来源:http://www.hnsfk.com 浏览: 发布日期:2018-05-28
    1、安全柜应放置于十万级以下的初级净化间。操作前应打开紫外灯,照射30min,后将本次操作所需的全部物品移入安全柜,避免双臂频繁穿过气幕破坏气流;并且在移入前用75%酒精擦拭物品表面消毒,以去除污染。
    1, the safety cabinet should be placed in the primary purification room below grade one hundred thousand. Before operation, the ultraviolet lamp should be opened and 30min is irradiated. Then all the articles needed in this operation should be moved into the safety cabinet to avoid the frequent crossing of the arms through the air curtain to destroy the air flow; and the contamination is removed by the disinfection of the surface of the articles with 75% alcohol before moving in.
    2、打开风机10min,待柜内空气净化并气流稳定后再进行实验操作。将双臂缓缓伸入安全柜内,至少静止2min,使柜内气流稳定后再进行操作。
    2, turn on the fan 10min, then clean the air inside the cabinet and stabilize the airflow before proceeding the experiment operation. Slowly extend your arms into the safety cabinet, at least 2min at rest, so that the airflow in the cabinet can be stabilized before operating.
    3、生物安全柜内不放与本次实验无关的物品。物品应尽量靠后放置,不得挡住气道口,以免干扰气流正常流动。
    3, no matter in the biosafety cabinet is not related to this experiment. Objects should be placed as far back as possible so as not to block the air port, so as not to disturb the normal flow of air.
    4、操作时应避免交叉污染。为防止可能溅出的液滴,应准备好75%的酒精棉球或用消毒剂浸泡的小块纱布,避免用物品覆盖住安全柜的格栅。
    4. Cross pollution should be avoided in operation. To prevent possible splashing of liquid droplets, 75% of alcohol cotton balls or small gauze soaked with disinfectant should be prepared to avoid covering the safety cabinet with objects.
    5、在实验操作时,不可完全打开玻璃视窗,应保证操作人员的脸部在工作窗口之上。在柜内操作时动作应轻柔、舒缓,防止影响柜内气流。
    5. During the experimental operation, the glass windows should not be completely opened. The operator's face should be above the working window. The operation should be gentle and soothing in the cabinet to prevent air flow in the cabinet.
    6、安全柜应定期进行检测与保养,以保证其正常工作。工作中一旦发现安全柜工作异常,应立即停止工作,采取相应处理措施。
    6, safety cabinet should be regularly tested and maintained to ensure its normal work. Once the work of the safety cabinet is found to be abnormal in the work, the work shall be stopped immediately and corresponding measures shall be taken.
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