(1) The sterile room should be completely closed, and the entry and exit of the sterile room should pass through at least two doors, with buffer compartments between them. An open and closed window should be set between the sterile room and the outside for the delivery of equipment.
(2) asepsis room must be kept clean and tidy. Workers entering the asepsis room should exchange special shoes and special clothes. The sterile room should be disinfected with ultraviolet rays for 30 minutes before use, and the table surface should be cleaned after operation, and then disinfected with ultraviolet rays for 30 minutes. Regular use of lactic acid or formaldehyde fumigation, thorough disinfection.
(3) asepsis room is only used for aseptic operation such as media packing and so on. Operators should strictly close their doors and wear special respirators and hats.
(4) air filters should be installed in sterile rooms and air conditioners should be installed.
Two points need special attention in the operation of microbiological laboratory.
Owing to the requirement of aseptic operation, alcohol lamp is often used in the process of experiment, so ceiling fan can not be installed in microbiology laboratory. In order to meet the appropriate temperature for the experiment, especially to meet the requirements of temperature and humidity for some instruments, the laboratory should install air conditioning.
Pollutant treatment: the operating area must be equipped with a disinfecting cylinder to deal with contaminated items such as glass slides with live bacteria. The remaining specimens, used bacterial plates and test tubes should be placed safely in the centralized place, and then washed or discarded after disinfection and sterilization.
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