山东净之源净化工程有限公司

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  • 关于无菌室的管理你了解多少?
    来源:http://www.hnsfk.com 浏览: 发布日期:2019-08-09
      1. 建立无菌室管理制度。
      1. Establishment of aseptic room management system.
      2. 保持良好的卫生:相对湿度40%—60%,温度20—22℃,最好安装空调。
      2. Keep good hygiene: Relative humidity 40%-60%, temperature 20-22 C, it is best to install air conditioning.
      3.无菌室内不得进行其他工作,非手术物品不得放置于无菌室内,检查后的剩余样品不得存放。
      3. No other work shall be done in the sterile room, no non-surgical articles shall be placed in the sterile room, and no remaining samples after inspection shall be stored.
      4. 紫外线灯的使用寿命一般为300小时,到期应记录更换。
      4. Ultraviolet lamp's service life is generally 300 hours, which should be recorded and replaced when it expires.
      以菌落总数和大肠菌群数为例,说明了无菌实验过程
      Taking the total number of colonies and the number of coliforms as examples, the aseptic experiment process was explained.
      对测试台、手和测试设备进行消毒(确保无菌环境和无菌测试用品)。微生物生长的理想环境是无菌的,但我们应该尽力保护自己。必须穿戴防护服、口罩和手套。
      Disinfect test benches, hands and test equipment (ensure sterile environment and test equipment). The ideal environment for microbial growth is sterile, but we should try our best to protect ourselves. Protective clothing, masks and gloves must be worn.
      擦拭并消毒测试台,通常使用75%乙醇或新的消毒液。
      Wipe and disinfect the test bench, usually using 75% ethanol or new disinfectant.
      为了保持无菌,手、心和手腕都要消毒,因为它们很容易残留细菌。
      In order to remain sterile, hands, hearts and wrists should be disinfected because they are easy to retain bacteria.
      实验台上的药品、装有样品的盒子等实验工具应进行消毒。
      Pharmaceuticals on the test bench, boxes containing samples and other experimental tools should be disinfected.
    无菌室装修
      实验前检查培养基,无菌条件下称重
      Check the culture medium before the experiment and weigh it under aseptic condition.
      1)实验前,检查大肠菌群培养液的量及气道内是否有气泡。
      1) Before the experiment, check the amount of coliform culture medium and whether there are bubbles in the airway.
      2)打开超净风机,在无菌、气流缓慢的条件下工作。
      2) Turn on the super-clean fan and work under the condition of sterility and slow airflow.
      3)无菌称重时,最好戴手套。
      3) When weighing aseptically, gloves are best worn.
      4)接种针消毒
      4) Vaccination needle disinfection
      5)用细菌消毒开管口
      5) Disinfection of opening with bacteria
      6)细菌
      6) Bacteria
      当接种或向培养皿中倒入培养基时,请勿将培养皿底部接触试管口或瓶壁外,试管口或瓶口应倾斜,以便越过火焰。
      When inoculating or pouring the culture medium into the Petri dish, do not contact the bottom of the Petri dish with the mouth of the test tube or the outside of the bottle wall. The mouth of the test tube or the bottle should be tilted so as to cross the flame.
      微生物实验室工作人员必须有严格的无菌观念。很多实验都需要在无菌条件下进行,主要有以下几个原因:第一,在实验操作中防止人为污染样品;第二,确保工作人员的安全,防止因操作不当检测到致病菌而造成的人身污染。
      Microbiology laboratory staff must have strict aseptic concept. Many experiments need to be carried out under aseptic conditions, mainly for the following reasons: first, to prevent artificial contamination of samples in the experimental operation; second, to ensure the safety of staff and prevent personal contamination caused by improper detection of pathogenic bacteria.
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